智能化颗粒流料计量系统

在粮油、饲料、酿造、制药、化工、水泥、肥料等生产加工行业,对粒状、粉状物料进行连续精确地计量统计是这些行业生产过程科学化管理的重要环节。智能化颗粒流料计量系统是实现这一环节的重要手段。此系统的启用,淘汰了传统的人工磅秤、“数包”计量方式,大大减轻了人员劳动强度,统计效率高,速度快。由于系统采用了多种软、硬件措施,其累计计量精度远远优于0.2 % 。     

Immunocytochemical demonstration of calmodulin in cells secreting by exocytosis

Summary Calmodulin is a regulator of several calcium-dependent cellular processes. It has been suggested that it plays a role in the mechanism of secretion. Employing an indirect immunoperoxidase technique at the light microscope level, this study demonstrates the presence of calmodulin in several exocytotic cells (mast cells, thyroid follicular cells, neurohypophyseal neurosecretory terminals, pancreatic-cells and pancreatic acinus cells) in rat and man. The positive staining reaction for calmodulin was granular and at least in the case of rat mast cells it appeared to be associated with the granule membrane.     

HPLC法测定感冒退热颗粒中表告依春和连翘苷

建立HPLC法同时测定感冒退热颗粒中含表告依春和连翘苷的量,以有效控制其质量.测定条件如下:Ultimate XB-C18(5μm,4.6 mm×250 mm)为色谱柱,乙腈-0.02%磷酸水溶液(23∶77)为流动相,检测波长235 nm,流速:1.0 mL/min,柱温为35℃.结果表明,表告依春在0.010 63~0.05315μg范围内呈线性关系(R=0.999 9),加样回收率为98.50%,RSD值为0.51%(n=9);连翘苷在0.099 81~0.499 05μg范围内呈线性关系(R=0.999 9),加样回收率99.93%,RSD值为0.61%(n=9).不同生产厂家的感冒退热颗粒中含连翘苷量相差不大,但含表告依春量差异较大.本实验建立的方法简便、稳定、准确可靠,可用于感冒退热颗粒的质量控制.     

HPLC法测参附丹强心颗粒中丹参素钠质量浓度

建立了HPLC法测定参附丹强心颗粒中丹参素钠质量浓度的方法.样品经水超声提取后,采用HPLC法测定参附丹强心颗粒中丹参素钠质量浓度,采用C18色谱柱,甲醇-0.5%磷酸(10∶90)为流动相.检测波长280 nm,流速1.0 m L/min.结果表明,丹参素钠线性范围为9.44~198.24μg/m L,相关系数r=0.999 8,平均回收率为96.58%.样品处理方法合理,方法学考察符合定量要求,结果准确,可用于参附丹强心颗粒中丹参素钠质量浓度测定.     

柴黄颗粒中总皂苷含量的测定

目的:建立柴黄颗粒中柴胡总皂苷的含量测定方法。方法:采用紫外可见分光光度法,在波长545 nm处对样品中的总皂苷进行含量测定。结果:柴胡总皂苷在0.038～0.266(mg.mL-1)范围内有良好的线性关系(r=0.999 7),该法的平均加样回收率为97.6%,RSD为1.8%(n=6)。结论:所建立的方法准确、简便、快速,重现性好,适合用于测定柴黄颗粒中总皂苷的含量,可为柴黄颗粒的质量控制提供依据。     

3种食物引诱剂诱螺效果及在灭螺剂中的作用

观察不同温度下3种食物引诱剂引诱钉螺的效果,并将其分别与灭螺植物绞股蓝水提物配伍制成颗粒灭螺剂进行灭螺实验,为研制既具有诱集作用,又具有毒杀作用的新型灭螺剂积累资料.结果表明:温度对食物引诱剂诱集钉螺有一定影响,在15-25℃诱集钉螺效果较好,35℃效果较差.在25℃实验条件下,H、M和C食物引诱剂24 h、48 h诱螺率分别为34.66%、79.34%;31.34%、56.34%;16.00%、38.66%.多因素方差分析表明:温度(F2,96=60.983,P<0.001)、食物引诱剂(F3,96=130.733,P<0.001)和时间(F3,96=103.040,P<0.001)对诱螺率都有很显著影响.3种食物引诱剂的诱螺效果依次为:H>M>C>CK(对照).用H、M、C 3种食物引诱剂分别制成颗粒灭螺剂诱杀钉螺,7 d钉螺死亡率分别为82.34%、66.34%、53.66%.     

Machine intelligence, rough sets and rough-fuzzy granular computing: uncertainty handling in bio-informatics and Web intelligence

Machine intelligence, is out of the system by the artificial intelligence shown. It is usually achieved by the average computer intelligence. Rough sets and Information Granules in uncertainty management and soft computing and granular computing is widely used in many fields,such as in protein sequence analysis and biobasis determination, TSM and Web service classification Etc.     

石韦配方颗粒HPLC-DAD特征图谱研究

为建立石韦配方颗粒HPLC-DAD特征图谱的测定方法,采用Inertsil~ODS-2C_(18)色谱柱(4.6mm×250mm,5μm),检测波长为265nm,柱温为30℃,流动相为甲醇-0.5%(体积分数)甲酸水溶液,梯度洗脱,流速为1.0mL/min,进样量为10μL。利用《中药色谱指纹图谱相似度评价系统》2012A对10个批次的石韦配方颗粒进行了相似度评价,并对其特征色谱峰峰面积进行了聚类分析。通过与标准品对比,确证了其中的2个色谱峰;通过多点校正及自动匹配,分析确定了9个特征峰;10批石韦配方颗粒的特征图谱相似度均大于0.85,通过聚类分析可将样品分为3类。该方法准确可靠,重复性好,可用于石韦配方颗粒的质量控制。     

Macro- and microscopic morphology of the reproductive system of Leptinaria unilamellata (d’Orbigny, 1835) (Mollusca: Pulmonata: Subulinidae)

Many tropical terrestrial gastropods, such as Subulinidae, are recognized and classified by their shells, as is the case for Leptinaria unilamellata (d’Orbigny, 1835), a hermaphroditic pulmonate snail restricted to tropical America. We aimed to characterize the morpho-anatomy and histology of the reproductive system of L. unilamellata. We compared the results obtained from L. unilamellata with the available data on the reproductive system of other subulinid species. The main distinctive characters are those of the penial complex, such as the proportion of the length of the penial complex and the length of the free oviduct, presence of a flagellum, site of insertion of the bursa copulatrix duct relative to the site of insertion of the penial complex and presence and extension of the penial sheath.     

木霉菌水分散粒剂防治小麦纹枯病的田间药效研究

通过田间试验，检测木霉菌水分散粒剂（1×108 cfu/g）防治小麦纹枯病的效果。结果表明，木霉菌剂拌种和灌根的防治效果达到71.59%，增产率为16.8%，均明显高于对照药剂20%井冈霉素可溶性粉剂，对环境安全、选择性高且不易产生抗药性。